Catalog Number: 1760420, 1760426
Phosphate Buffered Saline (PBS), Dulbecco's Formula, powder, without calcium, magnesium

Dissolve one packet of powder in 1 L of distilled water. The pH will be in the range of 7.3 - 7.5. If desired, add sodium bicarbonate. The pH can be adjusted as desired using 1N HCl or 1N NaOH. Sterile filter, if desired. Store liquid at 2 - 8oC.


Formulation:
Components
mg/L
Mol. Wt.
Mol. (mM)
Inorganic Salts
Potassium Chloride [KCl]
200.0000
74.55
2.68
Potassium Phosphate Monobasic [KH2PO4]
200.0000
136.09
1.47
Sodium Chloride [NaCl]
8000.0000
58.44
136.89
Sodium Phosphate Dibasic [Na2HPO4]
1150.0000
141.96
8.10

Availability:

1X Liquid:

Earle's Balanced Salts (EBS), Modified:
Catalog NumberDescriptionSize
18000491X EBS (Modified)100 ml
1800054500 ml
18002541X EBS (Modified) without phenol red500 ml
18004491X EBS (Modified), without calcium, magnesium100 ml
1800454500 ml
190015410X EBS (Modified), without sodium bicarbonate500 ml
1700122Powdered, EBS (Modified), without sodium bicarbonate1 x 10 liter

Phosphate Buffered Saline (PBS):
Catalog NumberDescriptionSize
18600491X PBS100 ml
1860054500 ml
18604491X PBS without calcium, magnesium100 ml
1860454500 ml
18610541X PBS with magnesium chloride, without magnesium sulfate500 ml

Hanks' Balanced Salts (HBS), Modified:
Catalog NumberDescriptionSize
18100491X HBS (Modified)100 ml
1810054500 ml
18102541X HBS (Modified) without phenol red500 ml
18104491X HBS (Modified), without calcium, magnesium100 ml
1810454500 ml
18105541X HBS (Modified), without calcium, magnesium, phenol red500 ml

10X Liquid:

Earle's Balanced Salts (EBS), Modified:
Catalog NumberDescriptionSize
190015410X EBS (Modified), without sodium bicarbonate500 ml

Phosphate Buffered Saline (PBS):
Catalog NumberDescriptionSize
196005410X PBS500 ml
196044910X PBS without calcium, magnesium100 ml
1960454500 ml
196105410X PBS with magnesium chloride, without magnesium sulfate500 ml

Hanks' Balanced Salts (HBS), Modified:
Catalog NumberDescriptionSize
191014910X HBS (Modified) without sodium bicarbonate100 ml
1910154500 ml
191034910X HBS (Modified) without sodium bicarbonate, phenol red100 ml
191065410X HBS (Modified), without calcium, magnesium, sodium bicarbonate500 ml

Powders:

Earle's Balanced Salts (EBS), Modified:
Catalog NumberDescriptionSize
1700122Powdered, EBS (Modified), without sodium bicarbonate1 x 10 liter

Phosphate Buffered Saline (PBS):
Catalog NumberDescriptionSize
1760020PBS, powder, with calcium, magnesium10 x 1 liter
17600221 x 10 liter
1760420
1760426
PBS, powder, without calcium, magnesium10 x 1 liter
1 x 1 liter

Hanks' Balanced Salts (HBS), Modified:
Catalog NumberDescriptionSize
1710120HBS (Modified), powder, without sodium bicarbonate10 x 1 liter
17101221 x 10 liter
17101241 x 50 liter

Tablets:

Phosphate Buffered Saline (PBS):
Catalog NumberDescriptionSize
2810305
2810306
2810307
PBS tablets, without calcium, magnesium (1 tablet makes 100 ml)100 tab
200 tab
500 tab


References:

  1. Dulbecco, R., Vogt, M., J. Exp. Med., v. 99, 167 (1954)
  2. Paul, J., Cell and Tissue Culture, Livingstone, LTD., London (1959)